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一般說明
木瓜蛋白酶是一种天然存在于木瓜(Carica papaya L.)中的蛋白水解酶。可以从切开的未成熟木瓜的乳胶中获得。木瓜蛋白酶属于半胱氨酸蛋白酶家族。它是一种球状单链蛋白质,由四个二硫桥和催化基础残基组成。木瓜蛋白酶由全α-结构域和反平行片β-结构域组成。
應用
从番木瓜提取的木瓜蛋白酶已用于:
- 酶解大鼠皮质以提取用于细胞培养的星形胶质细胞
- 研究对羊驼精浆粘度和精子功能的影响
- 研究对犀牛精子产量和低温存活率的影响
木瓜蛋白酶被用作嫩肉精。它还被用于在原代细胞培养物制备中分解组织。
生化/生理作用
木瓜蛋白酶对许多生物体的生物过程起重要作用。它对蛋白质、氨基酸酯和酰胺连接以及短链肽具有蛋白水解活性。木瓜蛋白酶倾向于切割碱性氨基酸如精氨酸、苯丙氨酸和赖氨酸的肽键。它在药物发现、制药、食品、烘焙和乳制品行业有广泛的应用。
木瓜蛋白酶是一种可切割肽键的半胱氨酸蛋白酶,并且已知可切割抗体的Fc部分。
單位定義
1 U对应于在pH 6.2和25°C下,每分钟水解1 μmol N-苯甲酰基-L-精氨酸乙酯(BAEE,货号 12880)所需的酶量
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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