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Merck
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重要文件

A6255

Sigma-Aldrich

α-淀粉酶 来源于猪胰腺

greener alternative

PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

同義詞:

PPA, al1,4 葡聚糖-4-葡萄糖水解酶,, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶

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About This Item

酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Porcine pancreas

品質等級

種類

Type I-A

形狀

saline suspension

比活性

≥1000 units/mg protein (E1%/280)

分子量

51-54 kDa

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

運輸包裝

wet ice

儲存溫度

2-8°C

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一般說明

分子质量:51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链(包含2个SH基团和4个二硫键)以及一个紧密结合的Ca2+ (稳定性所必需的)组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0,最适pH为7。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时,该产品经优化可提高能效和防止浪费。有关详细信息,请参阅《Biofiles》的文章

應用

α-淀粉酶用于水解α-连接的多糖α键,如淀粉和糖原。产品 A6255 来自猪胰腺,为I-A 型。Sigma 公司生产的α-淀粉酶已被用于各种植物研究,如 拟南芥 代谢研究。

生化/生理作用

α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4) 葡聚糖键。天然底物,如淀粉和糖原,被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白,由约 475 个残基的单一多肽链组成,含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca 2 +

單位定義

一个单位将在20℃的pH 6.9下,在3分钟内从淀粉中释放1.0毫克麦芽糖

外觀

含 3 mM CaCl 的 2.9 M NaCl 悬液

準備報告

PMSF处理。2×结晶

抑制劑

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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存取文件庫

J Ratseewo et al.
Food chemistry, 298, 124949-124949 (2019-07-02)
Starch digestibility and polyphenol content were investigated in six (white, red and purple) Thai rice varieties. Total phenolic content (TPC), total anthocyanin content (TAC), amylose content, gelatinization parameters and in vitro digestibility were determined. Purple and red rice varieties were
Joana Pico et al.
Food chemistry, 297, 124990-124990 (2019-06-30)
Use of banana flours as functional ingredients is growing due to their nutritional benefits derived from phenolics and dietary fiber. However, the effect oven-drying, freeze-drying and extrusion on the phenolic compounds or starch digestibility is not understood. In this work
Frederick J Warren et al.
Carbohydrate polymers, 117, 192-200 (2014-12-17)
In vitro hydrolysis assays are a key tool in understanding differences in rate and extent of digestion of starchy foods. They offer a greater degree of simplicity and flexibility than dynamic in vitro models or in vivo experiments for quantifiable
Yogesh Kumar Ahlawat et al.
Frontiers in plant science, 12, 762067-762067 (2021-11-20)
The precise role of KNAT7 transcription factors (TFs) in regulating secondary cell wall (SCW) biosynthesis in poplars has remained unknown, while our understanding of KNAT7 functions in other plants is continuously evolving. To study the impact of genetic modifications of
Yanhua Wang et al.
Journal of agricultural and food chemistry, 67(29), 8212-8226 (2019-07-17)
The factors that determine the digestion rate of starches were revealed using different forms of starches and a mixture of α-amylase and amyloglucosidase. Gelatinized starch samples with a degree of gelatinization (DG) from 12.2 to 100% for potato starch and

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Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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