推薦產品
品質等級
產品線
BioReagent
形狀
powder
成份
Dye content, ~80%
技術
nucleic acid detection: suitable
溶解度
ethanol: 2 mg/mL
4 mg/mL (2-methoxyethanol (EGME))
water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
適合性
suitable for flow cytometry
suitable for microscopy
儲存溫度
room temp
SMILES 字串
Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
InChI
1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
InChI 密鑰
RAHGLSRJKRXOSY-UHFFFAOYSA-L
尋找類似的產品? 前往 產品比較指南
一般說明
吖啶橙是一种异染性荧光阳离子染料,可渗透细胞膜并嵌入DNA和RNA。它能够实现在琼脂糖和聚丙烯酰胺凝胶上目视检测核酸。
應用
吖啶橙是一种细胞可渗透的异染色荧光阳离子染料,可嵌入 DNA 和 RNA,用于荧光和表观显微镜检测。吖啶橙染料已被用于通过流式细胞术分析线粒体和溶酶体含量,表征多药耐药性以及检测大鼠胸腺细胞凋亡过程中线粒体质量的变化。
适合
- 检测凝胶电泳分离的核酸
- 荧光和落射荧光显微镜
- 流式细胞术分析线粒体和溶酶体
- 凋亡研究中的DNA染色
特點和優勢
- 120 μM吖啶橙在单链和双链多核苷酸凝胶
- 差异染色的每个条带中可检测到25-50 ng的纯化DNA
原則
吖啶橙嵌入双螺旋核酸中,在530 nm处可检测到绿色荧光。它与单链核酸中的磷酸基团静电结合,在640 nm处可检测到红色荧光。
訊號詞
Warning
危險聲明
危險分類
Muta. 2
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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