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Key Documents

A1054

Sigma-Aldrich

抗-地高辛-碱性磷酸酶抗体,小鼠单克隆

clone DI-22, purified from hybridoma cell culture

同義詞:

单克隆抗-地高辛, 单克隆抗 地高辛 – 碱性磷酸酶 小鼠抗

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

重組細胞

expressed in mouse cell line

共軛

alkaline phosphatase conjugate

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

DI-22, monoclonal

形狀

buffered aqueous solution

技術

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

同型

IgG1

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

地高辛是一种强心苷,可用于治疗慢性心力衰竭。抗地高辛碱性磷酸酶单克隆抗体可用于检测地高辛标记的化合物,例如寡核苷酸、抗体和肽。该抗体对地高辛和地高辛标记的化合物具有特异性。该产品与地高辛具有很强的交叉反应性。

特異性

抗体对地高辛具有特异性,且对地高辛具有高亲和力。

免疫原

地高辛-KLH。

應用

巢式PCR产物可与5′端地高辛标记的寡核苷酸探针杂交。 向1 M磷酸盐缓冲液、1%牛血清白蛋白和0.1%吐温20(pH 7.4)中添加1:1000稀释的抗地高辛单克隆抗体碱性磷酸酶(DI-22)进行ELISA分析,以检测探针。使用Sigma Fass-Pnpp底物片剂进行检测。
抗地高辛碱性磷酸酶单克隆抗体适用于直接ELISA(1:10000-1:6000)和斑点杂交(以1:20,000稀释,使用地高辛标记的生物分子)。它还用于酶免疫分析(EIA)。

外觀

0.05 M Tris缓冲液,pH 8.0,含1% BSA、50%甘油、1 mM MgCl2和15 mM叠氮化钠

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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