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Merck
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重要文件

51411C

SAFC

最低必需培养基

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

製藥生產

同義詞:

Eagle最低必需培养基, MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

描述

for research or for further manufacturing use

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

成分

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Harry Eagle研究了小鼠L细胞和HeLa细胞在培养中的基本营养需求,因此开发了含有Earle′s平衡盐的最低必需培养基(MEM)。 他证明这些细胞需要13种氨基酸和7种维生素才能在体外生长和繁殖。 MEM是Eagle′s早期培养基基础培养基Eagle(BME)的改良,含有更高浓度的必需营养素。 这些培养基已证明能够在培养中支持多种正常细胞和转化细胞,并含有Earle′s平衡盐,这使其适合在装有CO2的大气中使用。

儲存類別代碼

12 - Non Combustible Liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Dirk M Reiter et al.
PLoS pathogens, 7(8), e1002166-e1002166 (2011-08-11)
Many viruses attach to target cells by binding to cell-surface glycans. To gain a better understanding of strategies used by viruses to engage carbohydrate receptors, we determined the crystal structures of reovirus attachment protein σ1 in complex with α-2,3-sialyllactose, α-2,6-sialyllactose
Michael J Tuvim et al.
PloS one, 4(1), e4176-e4176 (2009-01-13)
Influenza pneumonia causes high mortality every year, and pandemic episodes kill millions of people. Influenza-related mortality has been variously ascribed to an ineffective host response that fails to limit viral replication, an excessive host inflammatory response that results in lung
Wenxin Wu et al.
Virology, 396(2), 178-188 (2009-11-17)
We studied cytokine responses to influenza virus PR8 (H1N1) and Oklahoma/309/06 (OK/06, H3N2) in a novel human lung tissue model. Exposure of the model to influenza virus rapidly activated the mitogen-activated protein kinase signaling (MAPK) pathways ERK, p38 and JNK.
Chia-Chyi Liu et al.
PloS one, 3(3), e1810-e1810 (2008-03-20)
This work investigated the replication kinetics of the four dengue virus serotypes (DEN-1 to DEN-4), including dengue virus type 4 (DEN-4) recovered from an infectious cDNA clone, in Vero cells and in MRC-5 cells grown on Cytodex 1 microcarriers. DEN-1
Robert Nason et al.
Journal of the Association for Research in Otolaryngology : JARO, 10(2), 151-160 (2009-01-16)
Osteoclasts are the only cells capable of carrying out bone resorption and therefore are responsible for the osteolysis seen in infectious diseases such as chronic otitis media and infected cholesteatoma. Pseudomonas aeruginosa is the most common organism isolated from these

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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