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Key Documents

G6639

Sigma-Aldrich

异硫氰酸胍

≥97% (titration)

同義詞:

硫氰酸亚脲, 硫氰酸胍

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About This Item

線性公式:
NH2C(=NH)NH2 · HSCN
CAS號碼:
分子量::
118.16
Beilstein:
3563461
EC號碼:
MDL號碼:
分類程式碼代碼:
12352209
PubChem物質ID:
NACRES:
NA.55

生物源

synthetic

品質等級

化驗

≥97% (titration)

形狀

powder or crystals

技術

RNA extraction: suitable

顏色

white to off-white

mp

120-122 °C (lit.)

溶解度

water: 4 M, clear, colorless

負離子痕跡

chloride (Cl-): ≤2.5%

SMILES 字串

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI 密鑰

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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應用

硫氰酸胍可用于:
  • 用作变性剂研究其对球状蛋白表面活性的影响
  • 从原代肝细胞提取总核糖核酸(RNA)
  • 用作从人骨中提取DNA的培养缓冲液组分
  • 用作提取象鼻鲨的DNA的硫氰酸胍(GITC)裂解缓冲液组分
  • 用作从大鼠胰腺组织提取RNA的裂解液组分
离液剂和强效变性剂;溶解细胞。

生化/生理作用

硫氰酸胍(GTC)是一种强效蛋白质变性剂,能够变性内源性核糖核酸酶。它在从核糖体中分离核蛋白体核糖核酸(rRNA)方面起着重要作用。GTC可与苯酚、氯仿联用,从细胞和组织中分离总核糖核酸。

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

安全危害

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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Emilia Bramanti et al.
Analytical biochemistry, 351(1), 100-113 (2006-01-28)
In this article, a multidimensional dynamic surface tension detector (DSTD), in a parallel configuration with a UV-visible diode array absorbance detector, is presented in a novel flow injection analysis (FIA) application to study the effects of chemical denaturants urea, guanidinium
Julian R Peat et al.
F1000Research, 6, 526-526 (2017-06-06)
Methylation of CG dinucleotides constitutes a critical system of epigenetic memory in bony vertebrates, where it modulates gene expression and suppresses transposon activity. The genomes of studied vertebrates are pervasively hypermethylated, with the exception of regulatory elements such as transcription
Nicolas Chuvin et al.
Cellular and molecular gastroenterology and hepatology, 4(2), 263-282 (2017-07-29)
Transforming growth factor beta (TGFβ) acts either as a tumor suppressor or as an oncogene, depending on the cellular context and time of activation. TGFβ activates the canonical SMAD pathway through its interaction with the serine/threonine kinase type I and II
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Yuji Suzuki et al.
Bioscience, biotechnology, and biochemistry, 72(7), 1951-1953 (2008-07-08)
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds

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