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重要文件

MABF856

Sigma-Aldrich

Anti-Helios Antibody, clone 22F6

clone 22F6, from hamster(Armenian)

同義詞:

Zinc finger protein Helios, Ikaros family zinc finger protein 2, Helios

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

hamster (Armenian)

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

22F6, monoclonal

物種活性

human, mouse

技術

flow cytometry: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... IKZF2(22807)

一般說明

Zinc finger protein Helios (Uniprot Q9UKS7; also known as Ikaros family zinc finger protein 2, Zinc finger DNA binding protein Helios, Zinc finger protein subfamily 1A member 2) is encoded by the IKZF2 (also known as ANF1A2, HELIOS, ZNF1A2, ZNFN1A2) gene (Gene ID 22807) in human. Helios, Ikaros, Aiolos, Eos, and Pegasus constitute a family of transcription factors characterized by six highly conserved C2H2 zinc fingers. Their four N-terminal zinc fingers mediate DNA binding, while the two C-terminal zinc fingers are responsible for homodimeric and heterodimeric protein interactions. Eos and Pegasus are expressed in a wide variety of tissues, whereas Helios, Ikaros, and Aiolos are limited to the hematopoietic system. Specifically, Helios expression is reported to be restricted to a subpopulation (approximately 70%) of Foxp3(+) T cells in mice and humans.

特異性

Expected to react with isoform 1, 2 (Helios 1v), 3 (Helios del(Ex3,4)), 4 (Helios del(Ex6)v), 5 (Helios 1+2a,2b, Helios 1v+2a, Helios del(Ex3)+2a), 6 (Helios 1+5a, Helios 1+5a,5b), 7 (Helios del(Ex5)v), but not isoform 8 (Helios S).

免疫原

Epitope: N-terminal
Linear peptide corresponding to a human Helios N-terminal sequence.

應用

Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Helios in 1X10E6 human Jurkat cells.
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity among the majority of CXCR5-positive cells within the CD4+Foxp3+ population of murine peripheral lymph node (LN) cells (Chung, Y., et al. (2011). Nat Med.17(8):983-988).
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity in the Foxp3-positive, but not Foxp3-negative, subpopulation of CD4+ cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot revealed the age dependency of Helios-positive population among CD4+ murine splenocytes and CD4+/CD8- murine thymocytes (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot detected increased Helios-positive population among the TGF-beta-stimulated Treg, but not Teff cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Helios Antibody, clone 22F6 is validated for use in Flow Cytometry for the detection of Helios.

品質

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Helios in human PBMCs.

標靶描述

~ 59 kDa calculated

外觀

Protein G Purified
Format: Purified
Purified Armenian hamster monoclonal IgG antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells.
Thornton, AM; Korty, PE; Tran, DQ; Wohlfert, EA; Murray, PE; Belkaid, Y; Shevach, EM
Journal of immunology (Baltimore, Md. : 1950) (1950)
Yeonseok Chung et al.
Nature medicine, 17(8), 983-988 (2011-07-26)
Foxp3(+) regulatory T (T(reg)) cells suppress different types of immune responses to help maintain homeostasis in the body. How T(reg) cells regulate humoral immunity, including germinal center reactions, is unclear. Here we identify a subset of T(reg) cells expressing CXCR5

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