382180

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.

• 同義詞: HDAC6, Human, Recombinant
• 分類程式碼代碼: 12352202
• NACRES: NA.54

屬性

• 重組細胞: expressed in insect cells (S. frugiperda)
• 品質等級: 100
• 化驗: ≥80% (SDS-PAGE)
• 形狀: liquid
• 比活性: ≥50 units/μg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.

外觀

40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 3mM DTT, and 20% glycerol.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Pandey, U.B., et al. 2007. Nature447, 859.
Hubbert, C., et al. 2002. Nature417, 455.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1