AB3565

Anti-PARP (214/215) cleavage site Antibody

Chemicon^®, from rabbit

• 同義詞: Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, ARTD1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, bovine, rat, human
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NM_001618.3
• UniProt登錄號: P09874
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: bovine ... Parp1(286764) ; human ... PARP1(142) ; mouse ... Parp1(11545) ; rat ... Parp1(25591)

描述

一般說明

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

特異性

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.

The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

免疫原

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

應用

Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.

For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

品質

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

聯結

Replaces: 04-575; 04-576

外觀

ImmunoAffinity Purified

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

儲存和穩定性

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

分析報告

Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable