218807

Caspase-9, Human, Recombinant, E. coli

Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• 同義詞: ICE-LAP6, Mch6
• 分類程式碼代碼: 12352202

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 100
• 化驗: ≥70% (SDS-PAGE)
• 形狀: lyophilized
• 比活性: ≥400 units/mg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 溶解度: PBS with 15% glycerol: 1 units/μL
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.

外觀

Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄

重構

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.

其他說明

Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1