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Merck
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重要文件

C7482

Sigma-Aldrich

Caspase 6 human

recombinant, expressed in E. coli, >90% (SDS-PAGE), lyophilized powder, 13,000 units/mg protein

同義詞:

Mch2

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

化驗

>90% (SDS-PAGE)

形狀

lyophilized powder

比活性

13,000 units/mg protein

分子量

~30 kDa

溶解度

PBS: soluble

運輸包裝

dry ice

儲存溫度

−70°C

生化/生理作用

Cleaves the substrates consisting of consensus sequence VEID.
Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.

單位定義

One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 °C.

外觀

Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.

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分析證明 (COA)

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Ana A Baburamani et al.
Developmental neuroscience, 37(4-5), 321-337 (2015-04-01)
Apoptotic mechanisms are centre stage for the development of injury in the immature brain, and caspases have been shown to play a pivotal role during brain development and in response to injury. The inhibition of caspases using broad-spectrum agents such

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