C1099

半胱天冬酶 8 人

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine tagged), buffered aqueous solution, ≥1,000 units/mg protein

• 同義詞: FLICE, MASH, Mch5
• MDL號碼: MFCD01865996
• 分類程式碼代碼: 12352204
• NACRES: NA.32

屬性

• 重組細胞: expressed in E. coli (C-terminal histidine tagged)
• 品質等級: 200
• 化驗: ≥90% (SDS-PAGE)
• 形狀: buffered aqueous solution
• 比活性: ≥1,000 units/mg protein
• 分子量: ~30 kDa
• UniProt登錄號: Q14790
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CASP8(841)

描述

一般說明

Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag.

生化／生理作用

Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells.

單位定義

One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 °C.

外觀

Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS.

安全資訊

• 象形圖: GHS07,GHS08
• 訊號詞: Danger
• 危險聲明: H315 - H317 - H319 - H360D
• 防範說明: P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危險分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
• 儲存類別代碼: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable