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Sigma-Aldrich

REDExtract-N-Amp™ 种子 PCR 试剂盒

sufficient for 10 extractions, sufficient for 10 amplifications

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 10 amplifications
 sufficient for 10 extractions
 sufficient for 10 reactions

特點

dNTPs included
hotstart

技術

PCR: suitable

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

The REDExtract-N-Amp™ Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by direct PCR (polymerase chain reaction). A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.

應用

REDExtract-N-Amp™ seed PCR Kit has been used for DNA extraction

特點和優勢

  • Perfect for quick genotyping results
  • Rapid extraction of genomic DNA for PCR in 15 minutes
  • No long enzymatic digestions
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Compatible with a wide variety of seed sources
  • Extracts are stable at 4 °C for at least 6 months, allowing for multiple reassays
  • Phenol/chloroform extraction or alcohol precipitation not required
  • Column purification or centrifugation not required

原則

该试剂盒附带经验证的实验方案，可从玉米、小麦、向日葵、棉花、大豆、高粱、油菜和拟南芥种子中提取并扩增基因组DNA。可应要求提供其他实验方案。在常规操作步骤中，基因组DNA提取自研磨并在室温下的种子制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR Reaction Mix（包含在试剂盒内）的PCR反应物中，PCR反应即开始。

其他說明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律資訊

依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。依照美国专利号5,436,134，终端用户已自Molecular Probes, Inc.处获得仅在研究和开发用途实时定量PCR中使用SYBR Green核酸染色剂的许可。SYBR为Molecular Probes, Inc.的注册商标。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Seed Preparation Solution

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • E7526Extraction Solution化学品安全说明书
  • N3910Neutralization Solution B化学品安全说明书
  • R4775REDExtract-N-Amp™ PCR Reaction Mix化学品安全说明书

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

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商品

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

REDExtract-N-Amp™ Seed PCR Kit Protocol

The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).

REDExtract-N-Amp™ Seed PCR Kit Protocol

The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).

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