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Sigma-Aldrich

SYBR® Green 定量 RT-qPCR 试剂盒

One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

别名:

one step rt qPCR, sybr green qPCR

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选择尺寸

100 ML
US$174.24
500 ML
US$561.44

US$174.24


预计发货时间2025年4月26日



选择尺寸

变更视图
100 ML
US$174.24
500 ML
US$561.44

About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

US$174.24


预计发货时间2025年4月26日


品質等級

用途

sufficient for 250 reactions

特點

dNTPs included
hotstart

技術

RT-qPCR: suitable

顏色

colorless

輸入

purified RNA

相容性

ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000

檢測方法

SYBR® Green

運輸包裝

wet ice

儲存溫度

−20°C

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S9194KCQS00KCQS01
usage

sufficient for 250 reactions

usage

sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

colorless

color

colorless

color

colorless

color

colorless

input

purified RNA

input

purified DNA

input

purified DNA

input

purified DNA

compatibility

ABI 5700

compatibility

for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus

compatibility

for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480

compatibility

for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

一般說明

The SYBR® Green定量 RT-PCR 试剂盒将鼠白血病病毒(Moloney Murine Leukemia Virus)逆转录酶(M-MLV RT)、JumpStart Taq DNA聚合酶和SYBR Green I荧光染料组合成一步式 RT-PCR 试剂盒,设计用于检测基因表达。使用便捷的2X即用混合物包括M-MLV RT、SYBR Green I染料、JumpStart Taq DNA 聚合酶、99%纯度的脱氧核苷酸、缓冲液,玻璃钝化剂和稳定剂。 JumpStart Taq DNA聚合酶是一种抗体灭活的热启动酶(hot-start enzyme)。一旦反应温度达到70°C,DNA聚合酶-抗体复合物随即解离,Taq DNA聚合酶活性得以恢复。相对于手动热启动技术,这种抗体-酶复合物可轻松简便地准备,同时可降低污染风险。

應用

SYBR Green 定量 RT-PCR 试剂盒具有高特异性和再现性。 SYBR Green I是一种常用的荧光DNA结合染料,可结合所有双链DNA,并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。在加入一种热启动介导tag聚合酶,JumpStart Taq后,Sigma′s基于SYBR QPCR检测的特异性被增强。 该套件已经过优化,可与板/管实时仪器和罗氏 LightCycler 毛细管仪器一起使用。 一个单独的小瓶提供了参考染料,可用于 ABI 检测系统。
SYBR® Green定量 RT-qPCR 试剂盒用于一步式逆转录聚合酶链反应(RT-PCR)测定:
  • 检测诺如病毒基因组 I 和 II 的特定基因簇[1]
  • 定量测定基孔肯雅(CHIKV)RNA[2]检测 mRNA 表达水平[3][4]
  • 扩增从人脐静脉内皮细胞 (HUVEC)和[5] 前列腺癌细胞中提取的总 RNA

特點和優勢

  • 该预混液可确保反应间的一致性和可重现性
  • 缩短了制备时间,并降低了多个移液步骤造成的污染风险
  • 与手动或热启动(Hot Start)方法相比,减少了设置时间
  • JumpStartTaq聚合酶可减少引物二聚体和非特异性产物形成。
  • SYBR® Green I染料价格便宜、易于使用、灵敏度高
  • 广泛的仪器兼容性
  • 包括一个单独的ROX参考染料瓶,用于反应标准化

包裝

1个试剂盒足以用于每次50 μL的100次反应。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,994,056 和6,171,785。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何其它专利主张(如美国专利号6,814,934中的仪器或系统权利要求)下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利,包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。 如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系许可总监(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)获取。
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

仅试剂盒组分

产品编号
说明

  • SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included

  • M878725 mM MgCl2 1.5 mL/vial

  • R4526Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vial

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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    Carmela R Balistreri et al.
    Scientific reports, 9(1), 11028-11028 (2019-08-01)
    Bicuspid aortic valve (BAV) disease is recognized to be a syndrome with a complex and multifaceted pathophysiology. Its progression is modulated by diverse evolutionary conserved pathways, such as Notch-1 pathway. Emerging evidence is also highlighting the key role of TLR4
    Pei Jin Lim et al.
    PLoS neglected tropical diseases, 8(2), e2661-e2661 (2014-03-04)
    Chikungunya virus (CHIKV) has resulted in several outbreaks in the past six decades. The clinical symptoms of Chikungunya infection include fever, skin rash, arthralgia, and an increasing incidence of encephalitis. The re-emergence of CHIKV with more severe pathogenesis highlights its
    Gary P Richards et al.
    Applied and environmental microbiology, 70(12), 7179-7184 (2004-12-03)
    Genogroup I noroviruses from five genetic clusters and genogroup II noroviruses from eight genetic clusters were detected in stool extracts using degenerate primers and single-tube, real-time reverse transcription-PCR (RT-PCR) with SYBR Green detection. Two degenerate primer sets, designated MON 431-433
    Phui San Ho et al.
    Virology journal, 7, 13-13 (2010-01-23)
    Chikungunya virus (CHIKV) is a mosquito-borne alphavirus and one of the prevalent re-emerging arbovirus in tropical and subtropical regions of Asia, Africa, and Central and South America. It produces a spectrum of illness ranging from inapparent infection to moderate febrile
    Caiyun Shan et al.
    Molecular medicine reports, 17(4), 5300-5305 (2018-02-03)
    Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways

    商品

    RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

    Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

    One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

    A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.

    实验方案

    引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。

    Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

    Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

    Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.

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