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KCQS08

Sigma-Aldrich

KiCqStart®一步法探针RT-qPCR Readymix™

with Low ROX for ABI and Stratagene instruments

别名:

KiCqStart® One Step Probe RT-qPCR Readymix™

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 500 reactions

特點

dNTPs included
hotstart

儲存條件

protect from light

技術

qPCR: suitable

顏色

light blue

輸入

purified RNA

相容性

ABI 7500 Fast
 ABI 7500
 ABI QuantStudio
 ABI ViiA 7
 Agilent AriaMx
 Douglas Scientific IntelliQube
 Qiagen Corbett Rotor-Gene Q
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

檢測方法

probe-based

運輸包裝

dry ice

儲存溫度

−20°C

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相关类别

一般說明

KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.

KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.

應用

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Compatible instruments include:
  • Applied Biosystems® 7500
  • Applied Biosystems 7500 Fast
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio
  • Agilent® AriaMx
  • Douglas Scientific IntelliQube
  • QIAGEN® Rotor-Gene® Q
  • Stratagene Mx3000P®
  • Stratagene Mx3005P®
  • Stratagene Mx4000™

特點和優勢

  • 仅需33分钟即可获得检测结果
  • 高效和高灵敏度的实时PCR结果
  • 极少/无需优化

成分

2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume

法律資訊

Agilent is a registered trademark of Agilent Technologies, Inc.
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
Qiagen is a registered trademark of Qiagen Group
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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R Grace Walton et al.
Physiological reports, 3(6), doi:10-doi:10 (2015-06-04)
Reduced vessel density in adipose tissue and skeletal muscle is associated with obesity and may result in decreased perfusion, decreased oxygen consumption, and insulin resistance. In the presence of VEGFA, Angiopoietin-2 (Angpt2) and Angiopoietin-1 (Angpt1) are central determinants of angiogenesis
Tyler J Kirby et al.
Physiological genomics, 48(2), 145-153 (2015-12-17)
Adipose tissue has profound effects on whole-body insulin sensitivity. However, the underlying biological processes are quite complex and likely multifactorial. For instance, the adipose transcriptome is posttranscriptionally modulated by microRNAs, but the relationship between microRNAs and insulin sensitivity in humans
Tyler J Kirby et al.
Molecular biology of the cell, 27(5), 788-798 (2016-01-15)
Myofibers increase size and DNA content in response to a hypertrophic stimulus, thus providing a physiological model with which to study how these factors affect global transcription. Using 5-ethynyl uridine (EU) to metabolically label nascent RNA, we measured a sevenfold

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实验方案

KiCqStart™ One-Step Probe RT-qPCR ReadyMix™

KiCqStart One-Step Probe RT-qPCR ReadyMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan 5'-hydrolysis probes on real-time PCR systems.

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