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品質等級
形狀
liquid
用途
sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 400 reactions
特點
dNTPs included
hotstart
濃度
1.25 units/reaction (50 μL reaction volume)
技術
qPCR: suitable
顏色
colorless
輸入
purified DNA
適合性
suitable for PCR
應用
agriculture
相容性
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
檢測方法
SYBR® Green
運輸包裝
wet ice
儲存溫度
−20°C
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相关类别
一般說明
應用
- During RNA extraction, reverse transcription and Quantitative PCR
- During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)
- For tissue RNA Isolation. (PCR and melting curve analyses were performed)
- in real-time PCR (RT-PCR) of reverse-transcribed cDNA template
- For qPCR amplifications using SYBR
- For multiplex PCR
- to prepare the real-time PCR mixture
- in the quantitative analysis of gene expression by real time, quantitative polymerase chain reaction (qPCR)
- to determine the mRNA expression of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) gene by qPCR
特點和優勢
- 通过在ReadyMix中进行SYBR Green检测提供抗体灭活热启动PCR的优势，是高通量应用的理想之选；只需引物和模板即可
- JumpStart™ Taq DNA聚合酶可防止非特异性产物扩增，进而提高效率和靶标得率。
- SYBR® Green JumpStart® Taq ReadyMix适用于基于SYBR的qPCR，采用MgCl2配制或采用独立小瓶包装，便于优化。ReadyMixes与管式和板式仪器兼容
- 该预混液可确保反应间一致性
- 可最大限度减少污染
- 减少了引物二聚体
- 与手动或蜡质热启动®方法相比，制备时间更短
- 允许室温构建反应并随附荧光染料；qPCR应用的理想之选
包裝
20RXN包装为1 X 500μL
100RXN包装为1 X 2.5 mL
400RXN包装为1 X 10 mL
500RXN包装为1 X 12.5 mL
其他說明
原則
SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。 ROX参考染料仪器设定符合定量PCR参考染料测量需要。 在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。
法律資訊
相關產品
訊號詞
Warning
危險分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
商品
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
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