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S1816

Sigma-Aldrich

SYBR® Green JumpStart™ Taq ReadyMix™ 用于定量PCR，毛细管配制

SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特點

dNTPs included
hotstart

濃度

1 units/reaction (20 μL reaction volume)

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

相容性

for use with Roche LightCycler 480

檢測方法

SYBR® Green

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

SYBR® Green JumpStart™ Taq ReadyMix™预混液（毛细管配方）结合了热启动酶JumpStart Taq的优点，是专门设计用于毛细管仪器（例如Roche LightCycler®实时热循环仪）的2x浓缩预混液。 SYBR Green JumpStart Taq ReadyMix是一种优化配方，包含SYBR Green I染料、JumpStart Taq DNA聚合酶、99%纯脱氧核苷酸、缓冲液和稳定剂。

SYBR Green Taq ReadyMix可用于单个产品实时扩增实验，也可用于评价产生荧光标记探针之前的引物序列。 不建议将荧光标记探针与SYBR Green I染料一起使用。

應用

SYBR® Green JumpStart™ Taq ReadyMix™预混液（适合定量PCR，毛细管专用）可用于实时定量聚合酶链式反应(RT-qPCR)和逆转录PCR(RT-PCR)。

特點和優勢

  • 方便的2×浓缩预混液，专门设计用于毛细管仪器，例如Roche LightCycler®，是高通量应用的理想选择
  • 提高特异性&目标产量-JumpStart™ Taq聚合酶可防止非特异性产物，从而获得更准确的CT值并改善定量
  • 该预混液可确保反应间一致性
  • 缩短了制备时间，并降低了多个移液步骤造成的污染风险
  • SYBR® Green I荧光染料特异性结合双链DNA，低价、易用、灵敏，是任意DNA序列定量分析的理想选择

包裝

提供一管25 mM MgCl2，以便于优化QPCR反应。
默认反应体积为20 μL

100RXN包装为1 X 1 mL
400RXN包装为1 X 4 mL

原則

SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。

JumpStart Taq抗体在室温下能够使DNA聚合酶失活。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全被激活。JumpStart Taq DNA聚合酶防止非特异性扩增，能够获得更为准确的CT值。

为制备反应，将10 μL ReadyMix加入引物、模板和水中，使最终反应体积为20 μL。

單位定義

一个单位在74℃下可在30分钟内将10 nmol总dNTP融入酸可沉淀DNA中。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056 and 6,171,785.. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。我们未明确表示、暗示或以禁止反言的形式授予您其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LightCycler is a registered trademark of Roche
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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