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Merck
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文件

P2192

Sigma-Aldrich

10X PCR缓冲液

Optimized for routine PCR with MgCl2 included

别名:

PCR Buffer Solution, PCR Reaction Buffer

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About This Item

分類程式碼代碼:
41106306
NACRES:
NA.52

品質等級

300

形狀

liquid

包裝

vial of 1.5 mL

顏色

colorless

應用

agriculture

異物活動

DNase, RNase, none detected

儲存溫度

−20°C

應用

10X PCR缓冲液已用作PCR混合物组分：
  • 用于病毒DNA扩增
  • 扩增基因组DNA，研究注意缺陷多动障碍(ADHD)患者的亚甲基四氢叶酸还原酶(MTHFR)基因突变
  • 扩增芽孢杆菌来源的16S-23S rRNA基因内转录间隔区(ITS)

特點和優勢

  • 独立缓冲液
  • 可与JumpStart™ Taq DNA聚合酶（D9307）、来自栖热水生菌的Taq DNA聚合酶 （D1806）和REDTaq® 基因组DNA聚合酶（D8312）兼容

成分

10×缓冲液的组成：100mM Tris-HCl（pH8.3，25℃）；500mM KCl；15mM MgCl2；0.01％明胶

法律資訊

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Cem Gokcen et al.
International journal of medical sciences, 8(7), 523-528 (2011-09-08)
The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
Zhong-Dong Shi et al.
Stem cells translational medicine, 9(11), 1378-1388 (2020-07-15)
Human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells, hold great promise for cell-based therapies, but safety concerns that complicate consideration for routine clinical use remain. Installing a "safety switch" based on the inducible caspase-9
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实验方案

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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