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Merck

T8778

Sigma-Aldrich

海藻糖酶 来源于猪肾脏

buffered aqueous glycerol solution, ≥1.0 units/mg protein

别名:

αα-海藻糖-葡萄糖水解酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

buffered aqueous glycerol solution

品質等級

比活性

≥1.0 units/mg protein

濃度

0.5-10.0 mg/mL protein (BCA)

儲存溫度

−20°C

一般說明

海藻糖是大多数昆虫体内的主要血淋巴糖,是入侵病原真菌的主要靶标。
海藻糖酶(Trehalase)是一种糖苷酶,具有多种形式,存在于所有组织中。属于碳水化合物活性酶(CAZy)类中的糖苷水解酶37(GH37)家族成员。

應用

海藻糖已用于:
  • 评价葡萄霜霉病菌感染葡萄藤叶的糖代谢变化
  • 研究海藻糖-6-磷酸介导的生长停滞
  • 定量分析干酪乳杆菌Lactobacillus casei细胞内的海藻糖

生化/生理作用

海藻糖酶可催化水解一分子海藻糖生成两分子葡萄糖。它介导飞行代谢、蜕皮诱导的几丁质合成和低温耐受。

單位定義

一个单位将在37°C、pH 5.7 下每分钟将 1.0μmol 海藻糖转化为 2.0 μg葡萄糖(pH 7.5 下测定释放的葡萄糖)。

外觀

溶液溶于含 1%Triton X-100 和 25 mM 磷酸钾的 50% 甘油,pH 6.5

法律資訊

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Wim Schepers et al.
The Journal of biological chemistry, 287(53), 44130-44142 (2012-11-17)
The readdition of an essential nutrient to starved, fermenting cells of the yeast Saccharomyces cerevisiae triggers rapid activation of the protein kinase A (PKA) pathway. Trehalase is activated 5-10-fold within minutes and has been used as a convenient reporter for
Choowong Auesukaree et al.
Journal of bioscience and bioengineering, 114(2), 144-149 (2012-05-15)
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723
Dimitri A Skandalis et al.
Physiological and biochemical zoology : PBZ, 85(6), 657-670 (2012-10-27)
Although intraspecific variation in metabolic rate is associated with variation in body size, similarly sized individuals nonetheless vary greatly. At similar masses, hovering bumblebee workers (Bombus impatiens) can differ in metabolic rate up to twofold. We examined how such interindividual
Shari Dhaene et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1964-1989 (2020-11-10)
Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose

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