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Merck

T7408

Sigma-Aldrich

三乙基碳酸氢铵缓冲液

1.0 M, pH 8.5±0.1

别名:

三乙胺碳酸氢盐缓冲液

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About This Item

CAS号:
Beilstein:
3624751
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

形狀

liquid

儲存條件

(Tightly closed)

濃度

1.0 M

顏色

colorless

pH值

8.5±0.1

密度

1.02 g/mL at 25 °C (lit.)

適合性

suitable for LC-MS
suitable for mass spectrometry

應用

diagnostic assay manufacturing
microbiology
sample preparation

儲存溫度

2-8°C

SMILES 字串

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI 密鑰

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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應用

碳酸氢三乙铵缓冲液用于离子交换色谱和电泳。
还可用于:
  • 胰蛋白酶消化
  • 用作裂解缓冲液的组分
  • 调节缓冲液的pH值,以进行蛋白定量

用于离子交换色谱和电泳的缓冲剂。

生化/生理作用

碳酸氢三乙铵缓冲液可用于寡核苷酸纯化,以便进行电喷雾质谱分析。这种成分还可用于胰蛋白酶消化缓冲液。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

同類產品

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
Hao P, et al.
Journal of Proteome Research, 14(2), 1308-1314 (2015)
Dwayne A Elias et al.
Nucleic acids research, 37(9), 2926-2939 (2009-03-19)
Hypothetical (HyP) and conserved HyP genes account for >30% of sequenced bacterial genomes. For the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough, 347 of the 3634 genes were annotated as conserved HyP (9.5%) along with 887 HyP genes (24.4%). Given the large
C G Huber et al.
Journal of mass spectrometry : JMS, 35(7), 870-877 (2000-08-10)
The applicability of ion-pair reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry (IP-RP-HPLC/ESI-MS) and direct infusion/ESI-MS to the characterization of nucleic acid mixtures was evaluated by the analysis of the reaction products obtained from solid-phase synthesis of a 39-mer oligonucleotide. IP-RP-HPLC/ESI-MS

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