推荐产品
生物源
rat
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3F10, monoclonal
形狀
lyophilized
包裝
pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)
製造商/商標名
Roche
同型
IgG1
表位序列
YPYDVPDYA
儲存溫度
2-8°C
一般說明
抗-HA高亲和是一种HA-肽链(克隆3F10)的单克隆抗体。抗-HA高亲和识别HA肽链序列(YPYDVPDYA),这一序列来自人类流感血凝素蛋白。即使HA肽链表位通过表位标记,被引入不相关的重组蛋白,该抗体依然可以识别抗原决定簇。
特異性
抗-HA高亲和识别9-氨基酸序列(YPYDVPDYA),这一序列来自人类流感血凝素(HA)蛋白。该表位同样识别融合蛋白,无论它的位置在哪里(N端,C端或中间)。
免疫原
98-106氨基酸来自人类流感病毒血凝素蛋白
應用
使用抗-HA高亲和用于检测天然流感血凝素蛋白和含有HA表位的重组蛋白,可用方法有:
- 斑点印迹法
- ELISA
- 免疫细胞化学
- 免疫沉淀
- Western印迹法
品質
Western印迹法功能测试。
準備報告
工作浓度:偶联物工作浓度取决于用途和底物。
以下浓度可用作参考:
以下浓度可用作参考:
- ELISA:用于检测100ng/ml;用于涂层1至5μg/ml
- 免疫沉淀:0.5至5 μg/ml
- 蛋白质免疫印迹:50到200 ng/ml
重構
加入0.5ml(11 867 423 001; 50 μg)或2.5ml(11 867 431 001; 500 μg)双蒸馏水,使得终浓度分别为100 μg/ml或200 μg/ml。
使用前10分钟加水。
使用前10分钟加水。
其他說明
仅用于生命科学研究。不可用于诊断。
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訊號詞
Warning-Warning
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
does not flash
閃點(°C)
does not flash
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