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F3165

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

别名:

抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.32

生物源

mouse

品質等級

200

共軛

unconjugated

抗體表格

purified immunoglobulin (Purified IgG1 subclass)

抗體產品種類

primary antibodies

無性繁殖

M2, monoclonal

形狀

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

純化經由

using Protein A

物種活性

all

濃度

3.8-4.2 mg/mL

技術

western blot: 10 μg/mL (Protein A)

同型

IgG1

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

抗-Flag M2抗体用于检测Flag融合蛋白质。该单克隆产生于小鼠中并可识别FLAG序列的N-末端、Met N-末端和C-末端。该抗体还可在内部位点识别FLAG。不同于M1，M2不是钙依赖性的。
F3165使用蛋白质A树脂进行亲和纯化，因此不仅含有抗-FLAG M2抗体，还有少量的原生小鼠IgG，这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A

免疫原

FLAG；肽序列DYKDDDDK

應用

小鼠产生的 ANTI-FLAG® M2 单克隆抗体已用于：
  • 免疫印迹
  • 免疫沉淀
  • 免疫细胞化学
  • 免疫荧光
  • ELISA
  • EIA
  • 染色质免疫沉淀
  • 电子显微分析
  • 流式细胞分析
  • 高速迁移测定

浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。

準備報告

用3%脱脂牛奶在Tris缓冲盐溶液（TBS），pH 8.0中将抗体从0.5-10 ug/mL进行稀释

儲存和穩定性

将稀释后的抗体分装并-20°C储存。不建议反复冻融。
注意：随着时间流逝，由于分子间的疏水相互作用，少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀，请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Nature communications, 10(1), 3733-3733 (2019-08-21)
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Casey C Fowler et al.
Nature communications, 10(1), 3684-3684 (2019-08-17)
Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
Jeong Gu Kang et al.
Scientific reports, 9(1), 11960-11960 (2019-08-21)
Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but
Hong Zhu et al.
Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part
Annie M Sriramachandran et al.
Nature communications, 10(1), 3678-3678 (2019-08-17)
Modification with SUMO regulates many eukaryotic proteins. Down-regulation of sumoylated forms of proteins involves either their desumoylation, and hence recycling of the unmodified form, or their proteolytic targeting by ubiquitin ligases that recognize their SUMO modification (termed STUbL or ULS).
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