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Merck

CE0050

Sigma-Aldrich

CelLytic MEM 蛋白提取试剂盒

别名:

Membrane protein extraction kit

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About This Item

分類程式碼代碼:
41116134
NACRES:
NA.56

技術

protein extraction: suitable

品質等級

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

CelLytic MEM蛋白提取试剂盒提供了一种分离细胞中疏水性筏状微结构域相关蛋白的快速、方便的方法。该方法基于相分离,不需要分离细胞膜。分离的蛋白质可用于进一步的实验,例如 SDS-PAGE、蛋白质印迹、斑点印迹和免疫沉淀。 该试剂盒已在 HeLa、HEK-293、NIH 3T3、COS 和 CHO 及其它细胞系上进行了测试。
膜蛋白占据了生物体基因组大约20-30%的成分,充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能,例如将细胞与外部的毒素隔离开来,作为胞内信号级联的起始点,以及维持临界离子浓度。

應用

CelLytic MEM 蛋白质提取试剂盒已被用于以肝细胞测定脂肪从头 合成和提取用于蛋白质印迹的膜蛋白。

適合性

提供足够进行80次检测的试剂。

法律資訊

CelLytic is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • Lysis and Separation Buffer 50 mL

  • Wash Buffer for CelLytic MEM 50 mL

  • Sodium Chloride, 4M Solution 1.5 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL化学品安全说明书

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


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Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
Tomoaki Furuta et al.
Cancer science, 112(9), 3722-3731 (2021-06-12)
The rBC2LCN lectin, known as a stem cell marker probe that binds to an H type 3 fucosylated trisaccharide motif, was recently revealed to also bind to pancreatic ductal adenocarcinoma (PDAC) cells. A lectin-drug conjugate was generated by fusing rBC2LCN
Willy Morelle et al.
The Journal of clinical endocrinology and metabolism, 102(4), 1375-1386 (2017-03-23)
TMEM165 deficiency is a severe multisystem disease that manifests with metabolic, endocrine, and skeletal involvement. It leads to one type of congenital disorders of glycosylation (CDG), a rapidly growing group of inherited diseases in which the glycosylation process is altered.
Yue Zhao et al.
Cancer medicine, 7(8), 3977-3987 (2018-07-06)
Esophageal squamous cell carcinoma (ESCC) is a malignant disease with poor prognosis. Because of early metastasis prior to diagnosis and therapeutic resistance, ESCC has become one of the leading causes of cancer-related death. Here, we investigated the clinicopathological significance of
Emily C Cokorinos et al.
Cell metabolism, 25(5), 1147-1159 (2017-05-04)
The AMP-activated protein kinase (AMPK) is a potential therapeutic target for metabolic diseases based on its reported actions in the liver and skeletal muscle. We evaluated two distinct direct activators of AMPK: a non-selective activator of all AMPK complexes, PF-739

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