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描述
protease free
品質等級
形狀
liquid
包裝
pkg of 50 mL
pkg of 500 mL
技術
immunoprecipitation (IP): suitable
顏色
colorless
pH值
7.8-8.2 (25 °C, 1 ×)
儲存溫度
2-8°C
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一般說明
RIPA（放射免疫沉淀测定）缓冲液是一种无需准备即可使用的即用型溶液。其可最大限度避免非特异性蛋白质结合相互作用，可维持较低的背景，同时支持实现最具特异性的相互作用，从而可以研究相关蛋白质互作。由于大多数抗体和蛋白质抗原不受该缓冲液成分影响，这种成分广泛用于免疫沉淀等应用。
放射免疫沉淀检测缓冲液（RIPA缓冲液）是一种裂解缓冲液，用于从贴壁和悬浮培养的哺乳动物细胞中快速、有效地进行细胞裂解和蛋白质溶解。
應用
RIPA缓冲液已被用作以下实验研究中的细胞裂解试剂：
- 基于成纤维细胞的外周组织深度表型分型，有助于对零星帕金森病(sPD)进行机制性疾病分层。
- 验证氧化磷酸化 (OXPHOS)的抑制作用，这种成分可激活人肺上皮细胞的混合谱系激酶域样蛋白(MLKL)依赖性坏死。
RIPA缓冲液能够有效地进行细胞裂解和蛋白质溶解，并同时避免蛋白质的降解以及对蛋白质的免疫反应性和生物活性干扰。 RIPA缓冲液还可在免疫沉淀和分子沉淀检测中造成低的背景。 与EZview™亲和凝胶兼容。
特點和優勢
1 mL RIPA缓冲液可在100 mm培养皿（0.5至5 * 107个细胞）中裂解大多数粘附的哺乳动物细胞系细胞。
将非特异性的蛋白结合互作降低至最低以确保低的背景。
其他說明
含有150 mM NaCl、1.0% IGEPAL® CA-630、0.5%脱氧胆酸钠、0.1% SDS、50 mM Tris, pH 8.0的即用型溶液。
法律資訊
EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay
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产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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