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Merck
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文件

70584-M

Millipore

BugBuster®蛋白提取试剂

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About This Item

分類程式碼代碼:
41106516

形狀

liquid

品質等級

100

製造商／商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

儲存溫度

10-30°C

相关类别

一般說明

BugBuster®蛋白提取试剂是为温和地破坏大肠杆菌细胞壁并释放活性蛋白而配制的。它提供了一种简单、快速、低成本的替代机械方法，如弗氏压碎器或超声波法，用于释放表达的目标蛋白，以制备纯化或其他应用。该专利配方采用非离子和两性离子洗涤剂的三缓冲基混合物，能够在蛋白不变性的情况下完成细胞壁穿孔。BugBuster还可用于制备高纯度包涵体，用于表达的蛋白质不溶的情况下进行后续纯化。

應用

BugBuster®蛋白提取试剂已用于：
  • 裂解大肠杆菌BLR (DE3)细胞制备β-葡萄糖苷酶(BglB)并纯化
  • 作为细胞裂解缓冲液，处理聚囊藻PCC6803转偶联物，并测定黄色荧光蛋白(YFP)的荧光
  • 谷胱甘肽-转移酶-热休克蛋白家族A (Hsp70)成员8 (GST- HSPA8)的裂解缓冲液

警告

毒性：标准处理（A）

法律資訊

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

商品

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This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

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