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Merck
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文件

F7425

Millipore

抗-FLAG® 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

抗-ddddk, 抗-dykddddk

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About This Item

MDL號碼:
MFCD02262912
分類程式碼代碼:
12352203
NACRES:
NA.32

生物源

rabbit

品質等級

200

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

all

濃度

~0.8 mg/mL

技術

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

同型

IgG

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。
兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

免疫原

FLAG；肽序列DYKDDDDK

應用

采用斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测，该抗体可以识别N-末端或C-末端FLAG标记融合蛋白上的FLAG 表位。
请访问我们的 FLAG® 应用门户网站，了解更多产品详情。

生化／生理作用

FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

外觀

含有1％牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水（pH7.4）中的溶液

準備報告

在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

其他說明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Ryota Sato et al.
Frontiers in microbiology, 10, 1857-1857 (2019-08-29)
The envelope proteins of influenza A virus, hemagglutinin (HA) and neuraminidase (NA), play critical roles in viral entry to host cells and release from the cells, respectively. After protein synthesis, they are transported from the trans-Golgi network (TGN) to the
Makoto Miyazawa et al.
The Journal of biological chemistry, 286(22), 19191-19203 (2011-04-12)
The molecular chaperone prefoldin (PFD) is a complex comprised of six different subunits, PFD1-PFD6, and delivers newly synthesized unfolded proteins to cytosolic chaperonin TRiC/CCT to facilitate the folding of proteins. PFD subunits also have functions different from the function of
Martin S Taylor et al.
The Journal of biological chemistry, 288(45), 32211-32228 (2013-09-21)
Ghrelin O-acyltransferase (GOAT) is a polytopic integral membrane protein required for activation of ghrelin, a secreted metabolism-regulating peptide hormone. Although GOAT is a potential therapeutic target for the treatment of obesity and diabetes and plays a key role in other
Dorothee A Vogt et al.
PLoS pathogens, 9(4), e1003302-e1003302 (2013-04-18)
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous
Gouji Toyokawa et al.
Neoplasia (New York, N.Y.), 13(10), 887-898 (2011-10-27)
A number of histone methyltransferases have been identified and biochemically characterized, but the pathologic roles of their dysfunction in human diseases like cancer are not well understood. Here, we demonstrate that Wolf-Hirschhorn syndrome candidate 1 (WHSC1) plays important roles in
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