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Merck

R8508

Sigma-Aldrich

核糖核酸,转移 来源于面包酵母(酿酒酵母

buffered aqueous solution

别名:

tRNA, 转运 RNA

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352106
NACRES:
NA.55

等級

for molecular biology

品質等級

形狀

buffered aqueous solution

濃度

9-11 mg/mL

異物活動

DNase, Nickase, none detected

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

转移 RNA (tRNA) 是从面包酵母中分离得到的。
转运RNA(tRNA)是一条RNA核苷酸短链,呈L型。

應用

从面包酵母(S. cerevisiae)转移的核糖核酸适合用作核酸纯化和沉淀中的载体。
来自面包酵母 (S. cerevisiae)的转运核糖核酸可用于裸卵(denuded oocyte)mRNA沉淀和逆转录。还用于在鼻咽癌(NPC)细胞RNA pull-down实验中封闭磁珠。

生化/生理作用

转运RNA(tRNA)作为衔接分子将mRNA序列中的三核苷酸序列(密码子)翻译为对应的氨基酸序列。通过连接配对的氨基酸和核酸来确定遗传密码。tRNA参与病毒复制、细菌细胞壁生物合成、细胞应激反应和动物行为控制。tRNA分子异常与多种人类疾病有关。

成分

tRNA 溶于 1 mM EDTA 的 10 mM Tris HCl (pH 7.4) 溶液中。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Patrick Lonergan et al.
Molecular reproduction and development, 66(3), 297-305 (2003-09-23)
In the cyclic cow, final maturation of the ovulatory follicle is initiated by the preovulatory luteinizing hormone (LH) surge. During the subsequent 24 hr period, the oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization
Jean Lehmann et al.
Nucleic acids research, 41(10), 5494-5502 (2013-04-13)
The structure and function of conserved motifs constituting the apex of Stem I in T-box mRNA leaders are investigated. We point out that this apex shares striking similarities with the L1 stalk (helices 76-78) of the ribosome. A sequence and
Jin Chen et al.
Nature structural & molecular biology, 20(6), 718-727 (2013-04-30)
During translation elongation, the ribosome compositional factors elongation factor G (EF-G; encoded by fusA) and tRNA alternately bind to the ribosome to direct protein synthesis and regulate the conformation of the ribosome. Here, we use single-molecule fluorescence with zero-mode waveguides
Stefan Klumpp et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(42), 16754-16759 (2013-10-02)
Bacterial growth is crucially dependent on protein synthesis and thus on the cellular abundance of ribosomes and related proteins. Here, we show that the slow diffusion of the bulky tRNA complexes in the crowded cytoplasm imposes a physical limit on
Tanja Kalstrup et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(20), 8272-8277 (2013-05-01)
Atomic-scale models on the gating mechanism of voltage-gated potassium channels (Kv) are based on linear interpolations between static structures of their initial and final state derived from crystallography and molecular dynamics simulations, and, thus, lack dynamic structural information. The lack

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