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儲存溫度
−20°C
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一般說明
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）是RNase酶的底物。 从酵母中分离RNA是一个复杂的过程，涉及在苯和洗涤剂（SDS）环境中对细胞进行加热和冷冻循环。
應用
来自面包酵母（S. cerevisiae）的核糖核酸（RNA）适用于Northern印迹杂交、逆转录聚合酶链式反应（RTPCR）和cDNA构建等应用。
準備報告
通过 Crestfield, A.M.等人的修改版本制备，J. Biol. Chem., 216, 185 (1955)。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
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The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins.
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Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
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RBPmotif web server (http://www.rnamotif.org) implements tools to identify binding preferences of RNA-binding proteins (RBPs). Given a set of sequences that are known to be bound and unbound by the RBP of interest, RBPmotif provides two types of analysis: (i) de
实验方案
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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