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一般說明
Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.
應用
Ribonucleic acid, transfer from bovine liver has been used:
- to analyze the efficiency of thioflavin T (ThT) as a fluorophore
- to test its ability to inhibit the growth of endothelial cells
- as a component in translation mixture
- as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column
Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
生化／生理作用
Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.
單位定義
一单位在 1.0mL 水（1cm 光程）中的 A260 为 1.0。
分析報告
This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Shujuan Xu et al.
Scientific reports, 6, 24793-24793 (2016-04-22)
RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble
Molecular Biology of the Transfer RNA Revisited, 5-7 (2014)
Approaches for monitoring nuclear translation
mRNA Processing and Metabolism, 103-113 (2004)
RNA-Protein Interactions : A Practical Approach, 149-150 (1998)
From DNA to Protein: The Transfer of Genetic Information, 217-221 (1980)
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