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Merck

CK-RO

Roche

肌酸激酶(CK)

from rabbit muscle

别名:

肌酸磷酸激酶

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About This Item

分類程式碼代碼:
12352204

生物源

rabbit muscle

品質等級

形狀

lyophilized

包裝

pkg of 100 mg (10127566001)
pkg of 500 mg (10736988001)

製造商/商標名

Roche

顏色

white to light yellow

最適pH

6.0-7.0(reverse reaction)
9.0(forward reaction)

溶解度

water: soluble

適合性

suitable for immunoprecipitation

NCBI登錄號

UniProt登錄號

應用

life science and biopharma
sample preparation

異物活動

ATPase <0.001%
HK <0.001%
myokinase <0.001%

儲存溫度

2-8°C

基因資訊

rabbit ... CKM(100009056)

一般說明

at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.

應用

肌酸激酶已被用作体外翻译测定中使用的翻译缓冲液的组分,并且作为能量再生混合物的组分。

生化/生理作用

肌酸激酶(CK)是一种胍基激酶,消耗ATP催化肌酸可逆磷酸化为磷酸肌酸。它在具有高能量需求的细胞中快速再生ATP中起着至关重要的作用。人血液中CK水平的增加表明神经系统和心肌疾病、恶性低温和某些肿瘤。金属(例如Mg、Mn和Ca)充当CK的辅助因子。它还形成蛋白激酶C的底物,蛋白激酶C是参与细胞生长的重要信号分子。人们发现 CK的酶促反应产物,磷酸肌酸,可以阻止 HIV(人类免疫缺陷病毒)在巨噬细胞中复制。

品質

污染物:&lt;0.001% ATP酶,HK和肌激酶,每种

規格

具体活动:在+ 25°C下大约 350U/mg冻干物(+ 37°C下约800U/mg 冻干物),其中磷酸肌酸和ADP作为底物,并且N-乙酰基-L-半胱氨酸作为活化剂。

單位定義

一个单位CK活性(+ 25°C)= 2.3 U(+ 37°C),肌酸蛋白-P和ADP 作为底物,NAcCys作为重活化剂。

外觀

冻干粉,稳定

準備報告

储存条件(工作液):常规应用中的工作溶液和储存条件:
溶于0.1M 咪唑缓冲液中;等分并储存在- 15至-25°C。
在-15至-25°C稳定约四周。
避免反复冻融。

溶于0.5% NaHCO3溶液中。
不要冷冻。避光储存。
在2至8°C条件下储存时,稳定2-3天。

用含有0.2mM DTT的30mM甘氨酸缓冲液(pH9.0)溶解。
在2至8°C条件下储存时,稳定2-3天。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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Aurélie M Rakotondrafara et al.
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Irina M Kuznetsova et al.
Biochimica et biophysica acta, 1596(1), 138-155 (2002-05-02)
GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity and lifetime of bound ANS fluorescence.
J K Rao et al.
FEBS letters, 439(1-2), 133-137 (1998-12-16)
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 A resolution by X-ray diffraction methods, clearly identified the active site with bound sulfates surrounded by a constellation of arginine residues. The putative binding site of creatine, which is
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