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Merck
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Key Documents

R9001

Sigma-Aldrich

核糖核酸,转移 来源于面包酵母(酿酒酵母

Type X, lyophilized powder

别名:

tRNA, 转运 RNA

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
41106305
NACRES:
NA.55

等級

for molecular biology

種類

Type X

形狀

lyophilized powder

儲存溫度

−20°C

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一般說明

利用酚-氯仿抽提和乙醇沉淀法从面包酵母中分离出转移RNA (tRNA)。

應用

适合作为核酸纯化和沉淀中的载体使用。

成分

tRNA以冻干粉形式提供。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Mammalian in vitro splicing assays.
A Mayeda et al.
Methods in molecular biology (Clifton, N.J.), 118, 315-321 (1999-11-05)
Sean P Ryder et al.
Methods in molecular biology (Clifton, N.J.), 488, 99-115 (2008-11-05)
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to
Christophe Maris et al.
Nucleic acids research, 48(8), 4521-4537 (2020-03-15)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process, PTB promotes viral translation by interacting extensively with complex
A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.
Newton, S.S., et al.
Brain Res. Protoc., 9, 214-214 (2002)
Crystal E Fagan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S

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