推荐产品
生物源
bovine pancreas
品質等級
種類
Type II-A
形狀
solid
比活性
>= 60 Kunitz units/mg protein
分子量
~13,700
濃度
≥60% (SDS-PAGE)
技術
cell based assay: suitable
雜質
salt, essentially free
適合性
suitable for molecular biology
應用
diagnostic assay manufacturing
儲存溫度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI 密鑰
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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一般說明
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
核糖核酸酶不水解脱氧核糖核酸，因为脱氧核糖核酸缺乏对环状中间体的形成至关重要的2′-羟基。核糖核酸酶可以水解蛋白质样品中的核糖核酸。胰腺核糖核酸酶A在嘧啶（尿嘧啶或胞嘧啶）磷酸键的 3′-侧。
應用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
核糖核酸酶A可用于从DNA质粒制备物和蛋白质样品中除去RNA。核糖核酸酶A可用于RNA酶保护测定、去除非特异性结合的RNA、分析RNA序列、水解蛋白质样品中包含的RNA、以及DNA的纯化。来自牛胰腺的核糖核酸酶A已被用于评估人核糖核酸酶-1的杂合酶活性的研究中。 来自牛胰腺的核糖核酸酶A也已被用于研究阳离子交换蛋白置换色谱中基于颗粒的整体柱。
特點和優勢
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
準備報告
色谱纯化产品
分析報告
蛋白测定方法：E.
通过 SDS-PAGE 测定 RNase A 纯度
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Beata Schmidt et al.
Journal of chromatography. A, 1018(2), 155-167 (2003-11-19)
The overall topic of the investigation was the separation of basic proteins by cation exchange displacement chromatography. For this purpose two principal column morphologies were compared for the separation of ribonuclease A and alpha-chymotrypsinogen, two proteins found in the bovine
Elie Saliba et al.
eLife, 7 (2018-03-24)
The yeast Target of Rapamycin Complex 1 (TORC1) plays a central role in controlling growth. How amino acids and other nutrients stimulate its activity via the Rag/Gtr GTPases remains poorly understood. We here report that the signal triggering Rag/Gtr-dependent TORC1
Nana Asare et al.
Toxicology and applied pharmacology, 230(2), 175-186 (2008-04-18)
Mechanistic studies of nitro-PAHs (polycyclic aromatic hydrocarbons) of interest might help elucidate which chemical characteristics are most important in eliciting toxic effects. 1-Nitropyrene (1-NP) is the predominant nitrated PAH emitted in diesel exhaust. 1-NP-exposed Hepa1c1c7 cells exhibited marked changes in
Stephanie E Siegmund et al.
Human molecular genetics, 26(23), 4588-4605 (2017-10-04)
Mitochondrial disorders affecting oxidative phosphorylation (OxPhos) are caused by mutations in both the nuclear and mitochondrial genomes. One promising candidate for treatment is the drug rapamycin, which has been shown to extend lifespan in multiple animal models, and which was
Nicoletta Potenza et al.
Nucleic acids research, 34(10), 2906-2913 (2006-06-02)
Human ribonuclease-1 (hRNase-1) is an extracellular enzyme found in exocrine pancreas, blood, milk, saliva, urine and seminal plasma, which has been implicated in digestion of dietary RNA and in antiviral host defense. The enzyme is characterized by a high catalytic
实验方案
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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