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應用
来自 米曲霉的核糖核酸酶T1（RNase T1）可用于在测序之前对变性的RNA进行消化并用于蛋白折叠的研究 。
生化／生理作用
来自米曲霉的核糖核酸酶T1（RNase T1）是一种内切核糖核酸酶，其可在G残基后进行水解。切割发生在胍核糖核苷酸的3'-磷酸基团和相邻核苷酸的5'-羟基之间。初始产物为2'：3'环磷酸核苷，其可水解成相应的3'-核苷磷酸。它与胰腺RNase的不同之处在于它可特异性攻击鸟嘌呤位点以产生具有3′-GMP末端基团的3′-GMP和寡核苷酸。
單位定義
在pH7，5,37°C条件下，一个单位可在15分钟内产生相当于 ΔA260 为 1.0 的酸可溶性寡核苷酸，反应体积为1.0 mL。底物：酵母RNA。
外觀
2.8 M (NH4)2SO4 溶液混悬液
分析報告
E1%/280法测定蛋白
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
其他客户在看
Caroline Haupt et al.
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The journal of physical chemistry. B, 114(5), 1994-2003 (2010-01-22)
Because of their central importance for understanding enzymatic mechanisms, pK(a) values are of great interest in biochemical research. It is common practice to determine pK(a) values of amino acid residues in proteins from NMR or FTIR titration curves by determining
Patrizia Contursi et al.
Extremophiles : life under extreme conditions, 14(5), 453-463 (2010-08-25)
The pSSVx from Sulfolobus islandicus, strain REY15/4, is a hybrid between a plasmid and a fusellovirus. A systematic study previously performed revealed the presence of nine major transcripts, the expression of which was differentially and temporally regulated over the growth
Scott Quarrier et al.
RNA (New York, N.Y.), 16(6), 1108-1117 (2010-04-24)
Structure mapping experiments (using probes such as dimethyl sulfate [DMS], kethoxal, and T1 and V1 RNases) are used to determine the secondary structures of RNA molecules. The process is iterative, combining the results of several probes with constrained minimum free-energy
Colette M Castleberry et al.
Nucleic acids research, 38(16), e162-e162 (2010-07-01)
Transfer ribonucleic acids (tRNAs) are challenging to identify and quantify from unseparated mixtures. Our lab previously developed the signature digestion approach for identifying tRNAs without specific separation. Here we describe the combination of relative quantification via enzyme-mediated isotope labeling with
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Instructions for working with enzymes supplied as ammonium sulfate suspensions
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