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生物源
bakers yeast
品質等級
種類
Type III
形狀
ammonium sulfate suspension
比活性
≥400 units/mg protein (biuret)
分子量
tetramer 119,500 Da±600
濃度
≥3 mg/mL protein Biuret
技術
activity assay: suitable
顏色
white
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
yeast ... PGI1(852495)
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一般說明
研究领域：细胞信号传导
磷酸葡萄糖异构酶是一种普遍存在的多功能细胞质蛋白。该二聚体蛋白的分子量约为119,500 ± 600，由四个几乎相同的亚基组成。
磷酸葡萄糖异构酶是一种普遍存在的多功能细胞质蛋白。该二聚体蛋白的分子量约为119,500 ± 600，由四个几乎相同的亚基组成。
應用
来自面包酵母′（酿酒酵母）的磷酸葡萄糖异构酶的用途：
- 在血清甘露糖酶法测定中，用于将果糖-6-磷酸转化为葡萄糖-6-磷酸。
- 作为四酶之一，用于四酶联合反应测定磷酸甘露糖变位酶(PMM)活性 。
- 在葛根果糖含量测定中，将果糖转化为葡萄糖。
生化／生理作用
磷酸葡萄糖异构酶(PGI)对于D-葡萄糖-6-磷酸和D-果糖-6-磷酸的相互转化至关重要。PGI负责糖酵解的第二步，并参与葡萄糖生成和磷酸戊糖途径。它在细菌和真核生物中高度保守。PGI在烟曲霉(A. fumigatus)存活中起重要作用，在发育、细胞壁葡聚糖生物合成和毒力中发挥多样作用。磷酸葡萄糖异构酶可用作异构酶、神经白细胞素、自分泌运动因子，以及分化和成熟介质。
單位定義
一个酶活性单位是指在pH 7.4、温度25 °C条件下，每分钟将1.0 μm的D-果糖-6-磷酸转化为D-葡萄糖-6-磷酸所需的酶量。
外觀
2.6M (NH4)2SO4 中的晶体悬浮液，含 0.1mM 醋酸镁，pH 7.0
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
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Reproduction (Cambridge, England), 140(5), 721-732 (2010-08-19)
Glucose is a critical metabolic fuel in most mammals although many foodstuffs also contain high levels of the monosaccharides, galactose and fructose. The aims of this work were to determine the insulin response to challenges of these sugars (experiment 1)
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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