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生物源
bacterial (Leuconostoc mesenteroides)
品質等級
重組細胞
expressed in E. coli
形狀
ammonium sulfate suspension
比活性
≥550 units/mg protein (biuret)
分子量
128 kDa
儲存條件
(Tightly closed)
技術
cell culture | mammalian: suitable
UniProt登錄號
異物活動
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
儲存溫度
2-8°C
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一般說明
葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是一种糖蛋白，分子量为 128 kDa（凝胶过滤）。G6PD 是二聚体，每个亚基上有一个活性位点。
研究领域：细胞信号传导
研究领域：细胞信号传导
應用
葡萄糖-6-磷酸脱氢酶已用于：
- 测试玉米胚乳发育过程中的酮糖还原酶活性。
- 测定冠心病患者血清中甘露糖的水平
- 研究其对细胞外聚合物 (EPS) 提取物的反应活性，超声法测定细胞裂解液
- 测定培养人体肌肉卫星细胞的葡萄糖摄入情况
生化／生理作用
G6PD可为所有细胞提供还原动力（如烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)），使细胞能够平衡氧化压力。G6PD基因突变与 X 连锁G6PD缺陷有关——这是一种遗传性基因缺陷，包括新生儿黄疸和急性溶血性贫血等。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。
單位定義
在 NAD 存在下，在pH 7.8，30°C 下，1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。
外觀
用 3.2 M (NH 4 ) 2 SO 4 （含 50 mM Tris 和 1 mM MgCl 2 ，pH 7.5）混悬
其他說明
The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
其他客户在看
Cara A Griffiths et al.
Nature, 540(7634), 574-578 (2016-12-16)
The pressing global issue of food insecurity due to population growth, diminishing land and variable climate can only be addressed in agriculture by improving both maximum crop yield potential and resilience. Genetic modification is one potential solution, but has yet
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Mareike Hauenstein et al.
Bio-protocol, 7(18), e2561-e2561 (2017-09-20)
Hydroxylation of chlorophyll catabolites at the so-called C32 position ( Hauenstein et al., 2016 ) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
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