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形狀
lyophilized powder
比活性
≥100 units/mg solid
儲存溫度
−20°C
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一般說明
草酰乙酸脱羧酶 (OAD) 在厌氧菌中起 Na 泵的作用。是一种由 α、β 和 γ 三个亚基组成的膜蛋白，α 亚基含有羧化酶活性。
應用
来自假单胞菌 属的草酰乙酸脱羧酶已被用于消化低分子量（LMW）人乳馏分（5kF馏分）并可用作测定嗜热纤维梭状芽孢杆菌（C. thermocellum）草酰乙酸脱羧酶活性的阳性对照。
草酰乙酸脱羧酶已用于一项研究，以评估 Na (+)-谷氨酸盐转运蛋白 GltS 的转换率和折返环的可及性。 在一项研究中也被用来研究木葡糖酸醋杆菌对不同碳源的发酵和代谢特性。
生化／生理作用
草酰乙酸脱羧酶催化草酰乙酸盐的脱羧反应，其活性不需要锰和镁。它与多种革兰氏阴性菌有关。
單位定義
在 25℃、pH 8.0 的条件下，每分钟有 1 个单位的草酰乙酸转化为丙酮酸和 CO2
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Pius Dahinden et al.
Archives of microbiology, 182(5), 414-420 (2004-10-19)
Archaeoglobus fulgidus harbors three consecutive and one distantly located gene with similarity to the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae (KpOadGAB). The water-soluble carboxyl transferase (AfOadA) and the biotin protein (AfOadC) were readily synthesized in Escherichia coli, but the
Y Augagneur et al.
Journal of applied microbiology, 104(1), 260-268 (2007-10-12)
Citrate metabolism generates metabolic energy through the generation of a membrane potential and a pH gradient. The purpose of this work was to study the influence of oxaloacetate decarboxylase in citrate metabolism and intracellular pH maintenance in relation to acidic
Buvaneswari C Narayanan et al.
Biochemistry, 47(1), 167-182 (2007-12-18)
Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal
Dayanidhi Sarkar et al.
Applied microbiology and biotechnology, 87(1), 127-136 (2010-03-02)
The metabolism of Gluconacetobacter oboediens was investigated in relation to different carbon sources for the continuous cultures at the dilution rate of 0.05 h(-1). The 13C-flux result implies the formation of metabolic recycles for the case of using glucose and
Pius Dahinden et al.
Archives of microbiology, 183(2), 121-129 (2005-01-14)
The oxaloacetate decarboxylase (OAD) Na(+) pump consists of subunits alpha, beta, and gamma, which are expressed from an oadGAB gene cluster present in various anaerobic bacteria. Vibrio cholerae has two copies of oad genes, which are termed oad-1 and oad-2.
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