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Merck

G8255

Sigma-Aldrich

谷丙转氨酶 来源于猪心脏

lyophilized powder, ≥75 units/mg protein

别名:

L-丙氨酸:2-酮戊二酸氨基转移酶, GPT, 丙氨酸氨基转移酶(催化浓度)

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About This Item

CAS号:
Beilstein:
3562140
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Porcine heart

形狀

lyophilized powder

比活性

≥75 units/mg protein

成份

Protein, 75-95%

技術

cell culture | mammalian: suitable

顏色

light yellow to yellow

異物活動

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%

儲存溫度

−20°C

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一般說明

谷丙转氨酶(GPT)主要分布于肝脏。

研究领域:细胞信号传导

應用

猪心谷丙转氨酶已用于:
  • 检测其对人工合成的3-甲基-2-酮戊二酸外消旋混合物进行转氨反应的能力
  • 去除人胚肾细胞(HEK293)培养基中的谷氨酸
  • 研究乳酸脱氢酶(LD)-丙氨酸转氨酶(ALT)酶偶联反应系统的动力学

生化/生理作用

谷丙转氨酶(GPT)介导氨基从 l-丙氨酸转移到 α-酮戊二酸,从而产生丙酮酸和谷氨酸。因此,GPT 在糖异生和氨基酸代谢中发挥着关键作用。报道显示多种肿瘤中都有GPT参与。
谷丙转氨酶 (GPT) 存在于体内大多数细胞中,但在肝脏中浓度最高。由于在肝细胞坏死后 GPT 释放入血液,可作为肝脏疾病的标志物。

單位定義

一个单位在-37°C、pH 7.6且L-丙氨酸存在条件下将 1.0μmol 酮戊二酸转化成 L -谷氨酸。

外觀

含柠檬酸盐和醋酸盐的冻干粉

儲存和穩定性

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

分析報告

双缩脲法测定蛋白质

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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