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L3287

Sigma-Aldrich

Escort ™ IV 转染试剂

Lipid reagent for transient and stable transfection of mammalian and insect cells.

别名:

Gene delivery

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.25

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

1 mL sufficient for 160-500 transfections

濃度

1 mg/mL

技術

transfection: suitable

儲存溫度

2-8°C

相关类别

一般說明

Escort™ IV是由专利的聚阳离子脂质体和中性非转染脂质体构成的独特配方。这种脂质体构成的化合物可用于向不同类型的真核细胞转染核酸。

應用

适用于将核酸瞬时和稳定转染到培养的真核细胞中。 每 6 cm 细胞培养板使用约 4-16μL Escort™ IV 和 2μg DNA。方案优化提供了非常高效的转染。关于使用 Escort ™ IV 成功转染的细胞列表，请参见 转染试剂选择指南 。

特點和優勢

  • 适于稳定和瞬时转染
  • 针对广泛的细胞类型进行了优化
  • 低细胞毒性
  • 兼容含血清和不含血清的转染实验
  • 非常适于 Sf9, Sf21 和S2昆虫细胞

成分

Escort™ IV 配方:
1 mg/mL溶于水的总脂质体

请注意，Escort™ IV 中使用的脂质体具体类别属于机密信息。

注意

注意：不要冻存。

原則

在不存在血清条件下，将Escort™ IV与DNA混合，即可形成稳定的复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。

法律資訊

Escort is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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