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Merck
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Sigma-Aldrich

磷酸钙转染试剂盒

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

别名:

Gene delivery

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About This Item

EC號碼:
233-140-8
分類程式碼代碼:
12352200
NACRES:
NA.85

等級

for molecular biology

品質等級

200

形狀

solution

用途

 kit sufficient for 160 transfections (6 cm dishes)
 kit sufficient for 400 transfections (3.5 cm dishes)
 kit sufficient for 80 transfections (10 cm dishes)

技術

transfection: suitable

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

磷酸钙法转染是将DNA引入真核细胞的常用方法。这一技术已经在广泛的细胞类型中使用，可实现瞬时和稳定的转染效果。

應用

磷酸钙转染试剂盒用于：
  • 启动转染
  • 转染 Hek293T 细胞
  • 转染 H29D 细胞

适用于瞬时和稳定地将DNA转染至培养的哺乳动物细胞中。 使用磷酸钙方法已成功转染了以下细胞：

BAEC
肠黑素瘤细胞
CHO K1
COS-7
成纤维细胞（人胚胎、新皮肤）
HEK293
Huh7
IMR-90
LLC（Lewis肺癌）
NIH3T3
PC-12
PCI-13
SH-SY5Y
SK-HEP-1
T47D

特點和優勢

  • 适用于瞬时和稳定转染
  • 可重复用于多种细胞类型
  • 广泛引用
  • 价格便宜

成分

磷酸钙转染试剂盒包含：
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)

原則

该方法是基于将含有磷酸钠的HEPES缓冲盐水和含有DNA的CaCl2 溶液进行缓慢混合实现的。 所形成的DNA-磷酸钙共沉淀物可粘附于细胞表面，并可能通过胞吞作用被细胞吸收。

其他說明

该方案可用于使用pSV40-CAT质粒作为报告基因对CHO细胞进行瞬时转染。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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氯化钙 溶液 BioUltra, for molecular biology, ~1 M in H2O

Sigma-Aldrich

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氯化钙 溶液

X-tremeGENE™ 360 转染试剂 Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

Roche

XTG360-RO

X-tremeGENE™ 360 转染试剂

A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
A Calcium Phosphate Transfection
Ausubel, F. et al (ed.)
Short Protocols in Molecular Biology, 3rd, 9-9 (1995)
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in
M Wigler et al.
Cell, 14(3), 725-731 (1978-07-01)
Previous studies from our laboratories have demonstrated the feasibility of transferring the thymidine kinase (tk) gene from restriction endonuclease-generated fragments of herpes simplex virus (HSV) DNA to cultured mammalian cells. In this study, high molecular weight DNA from cells containing
Knock-down of SOX11 induces autotaxin-dependent increase in proliferation in vitro and more aggressive tumors in vivo
Conrotto P, et al.
Molecular Oncology, 5(6), 527-537 (2011)
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Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

实验方案

Calcium Phosphate Transfection Kit Protocol

Calcium Phosphate Transfection Kit Protocol

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