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NPT01

Sigma-Aldrich

NeuroPorter™转染试剂盒

Lipid formulation for nucleic acid transfections in neuronal and glial cells

别名:

NeuroPorter Transfection, Transfection Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.25

等級

for molecular biology

品質等級

200

形狀

dried film

用途

 kit sufficient for 75-200 transfections

存貨情形

available only in USA, Canada and EU

技術

transfection: suitable

儲存溫度

2-8°C

相关类别

一般說明

Neuroporter™ 转染试剂是经过优化的专利阳离子脂质体配方，毒性低，可用于向原代神经细胞、神经胶质细胞以及培养的神经细胞系之中高效递送DNA。Neuroporter™转染试剂盒可用于难转染原代神经细胞，可解决诸如细胞活力不足、转染效率低以及神经细胞退化等之前存在的问题。

應用

适于向原代神经细胞和培养的神经细胞系之中瞬时和稳定转染核酸。每一块6 cm细胞培养板可使用大约15-120 μl Neuroporter™的转染试剂和6-8 μg 的DNA（可应客户要求，以溶于独特的DNA稀释液的形式提供）。以下细胞已采用Neuroporter™转染试剂盒成功转染。

  • C6胶质瘤细胞（人类）
  • 皮质神经元细胞（大鼠原代细胞）
  • 背根神经节(DRG)细胞（大鼠）
  • NT2神经细胞（人前体细胞）
  • NT神经元（人分化细胞）
  • 室下区 (SVZ)细胞（小鼠）
  • 白质细胞（小鼠）

特點和優勢


  • 针对原代神经细胞、神经胶质细胞以及培养的神经细胞系进行了优化
  • 具有超低的细胞毒性，不会造成神经细胞退化或树突戒断
  • 可有效地递送原代神经细胞、神经胶质细胞以及培养的神经细胞系
  • 相对于其他方法，使用更为轻松、快捷
  • 兼容含血清和不含血清的转染实验

成分

1小瓶Neuroporter™ 转染试剂，为干燥的脂质体膜形式(T2823)
1.5 mL缓冲液 H9036
7.5 mL DNA稀释液D1941

注意

注意：不要冻存。

原則

在无血清的条件下混合Neuroporter™转染试剂和DNA，即可形成稳定的非共价偶联复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。

法律資訊

NeuroPorter is a trademark of Gene Therapy Systems, Inc.

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说明
价格

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCJ6956
SLCH2000
SLCC1660
SLBZ1116
SLBW7289

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Nikhil G Thaker et al.
Journal of neuroscience methods, 185(2), 204-212 (2009-09-29)
A major challenge for the treatment of cancers, such as glioblastoma multiforme (GBM), has been resistance to radiation and cancer chemotherapeutics. Short interfering RNA (siRNA) based screening may facilitate the identification of genes and pathways essential for cancer cell survival
Ling Luo et al.
eLife, 2, e00324-e00324 (2013-06-26)
Optimal phototransduction requires separation of the avascular photoreceptor layer from the adjacent vascularized inner retina and choroid. Breakdown of peri-photoreceptor vascular demarcation leads to retinal angiomatous proliferation or choroidal neovascularization, two variants of vascular invasion of the photoreceptor layer in
Adan Aguirre et al.
Nature, 467(7313), 323-327 (2010-09-17)
Specialized cellular microenvironments, or 'niches', modulate stem cell properties, including cell number, self-renewal and fate decisions. In the adult brain, niches that maintain a source of neural stem cells (NSCs) and neural progenitor cells (NPCs) are the subventricular zone (SVZ)
Beata Jablonska et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(42), 14775-14793 (2012-10-19)
Diffuse white matter injury (DWMI) caused by hypoxia is associated with permanent neurodevelopmental disabilities in preterm infants. The cellular and molecular mechanisms producing DWMI are poorly defined. Using a mouse model of neonatal hypoxia, we demonstrate a biphasic effect on
Simone Di Giovanni et al.
The Journal of biological chemistry, 280(3), 2084-2091 (2004-11-04)
Following spinal cord injury, there are numerous changes in gene expression that appear to contribute to either neurodegeneration or reparative processes. We utilized high density oligonucleotide microarrays to examine temporal gene profile changes after spinal cord injury in rats with
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商品

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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