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一般說明
MISSION® siRNA转染试剂适于转染siRNA到真核细胞，可实现≥90%的基因沉默达到瞬时基因敲除的效果。
使用低浓度的siRNA (1 nM)即可有效地沉默基因并降低脱靶效应。siRNA转染试剂以无菌溶液的形式提供，兼容血清和抗生素。
使用低浓度的siRNA (1 nM)即可有效地沉默基因并降低脱靶效应。siRNA转染试剂以无菌溶液的形式提供，兼容血清和抗生素。
其他說明
基因沉默实验：采用24孔板、8.4 ng的21-mer荧光素酶siRNA、2 μL MISSION® siRNA转染试剂转染稳定表达萤火虫荧光素酶的A549细胞系。在存在血清的条件下分成三等分开展实验。转染后48小时，对细胞裂解液之中的荧光素酶表达情况进行评估。
法律資訊
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
120.2 °F
閃點（°C）
49 °C
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This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
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CRISPR, miRNA, siRNA, and shRNA technologies for gene expression and gene silencing applications.
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
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