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一般說明
ITS+3液体培养基补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠、亚油酸和牛血清白蛋白(BSA)的混合物。适合无血清细胞培养应用。
應用
ITS+3液体培养基补充剂(100×)作为培养基补充剂已用于：
- 支持人脂肪干细胞(ASC)生长
- 培养猪椎间盘(IVD)细胞和组织
- 培养软骨终板(CEP)细胞
生化／生理作用
- 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，可能会影响有丝分裂。
- 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了滋养培养细胞，可与血清转铁蛋白结合后使用。
- 硒：血清中的一种必需微量元素。
成分
含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、470 μg/m亚油酸、470 μg/ml 油酸和50 mg/ml牛血清白蛋白（100x 浓度）。每摩尔白蛋白含有 2 摩尔亚油酸和油酸。
準備報告
使用不含酚红的Earle′平衡盐溶液（EBSS）制备。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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