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I2521

Sigma-Aldrich

ITS + 1 液体培养基补充剂 (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

Insulin-transferrin-sodium selenite, linoleic-BSA supplement

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About This Item

分類程式碼代碼:
12352205
NACRES:
NA.75

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

運輸包裝

ambient

儲存溫度

2-8°C

相关类别

一般說明

无血清培养可避免血清带来的成分差异和污染。无血清配方将通常由血清提供的因子替换为纯化形式，适合许多体外生长和分化研究。这些纯化因子包括胰岛素、转铁蛋白、硒、丙酮酸和乙醇胺。其他补充成分并不一定，具体取决于研究的细胞类型和所用的基础培养基。ITS补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠的混合物。它是一种通用的细胞补充剂，设计用于含有丙酮酸钠的非复合型培养基（如MEM、RPMI-1640）及复合型培养基（如Ham′s F-12、DME/F-12、MEM）。

應用

ITS+1液体培养基补充剂(100×)已用作高糖杜氏改良Eagle培养基(DMEM)的补充剂，用于小鼠间充质干细胞(mMSC)向软骨细胞的分化。还用作基础分化培养基的补充剂，用于在无血清条件下使人多能诱导干细胞(hiPSC)分化为成骨细胞。
胰岛素 - 转铁蛋白 - 亚硒酸钠，亚油酸 - BSA已用于无血清细胞培养应用.ITS + 1液体培养基补充剂（100×）已用于培养细胞系和用于制备永生化培养基，以用于生产来自′永生鼠′胚胎的细胞系。

成分

含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、50 mg/mL 牛血清白蛋白和 470μg/ml 亚油酸（100x 浓度）。

準備報告

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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