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Merck

L9655

Sigma-Aldrich

亚油酸-油酸-白蛋白,100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

Fatty Acid-Albumin-complexes

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About This Item

分類程式碼代碼:
12352211
NACRES:
NA.75

生物源

protein from bovine

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

成份

linoleic acid and oleic acid, 2 mol/mol albumin (each)

濃度

100 mg/mL BSA in DPBS

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

儲存溫度

2-8°C

一般說明

脂肪酸-白蛋白复合物在无血清培养基的研制中用作脂质的替代来源。其中脂肪酸以高配比与血清蛋白质相结合。这些蛋白质可以释放有益脂肪酸并结合那些有抑制作用的脂肪酸。油酸与牛血清白蛋白(BSA)结合有利于多种细胞类型(例如幼仓鼠肾 BHK)的生长。多不饱和和单不饱和脂肪酸(如,亚油酸和油酸)在用作培养基补充剂使用时会产生协同效应。

應用

亚油酸-油酸-白蛋白(100x)用于:
  • 诱导原代肝细胞中的急性肝细胞应激
  • 诱导滋养层细胞中的氧化应激
  • 诱导HepG2 细胞中的脂滴积聚

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说明
价格

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Pierluigi Ramadori et al.
Free radical biology & medicine, 91, 114-126 (2015-12-25)
Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))
Laura Moody et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)
Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley
Liang Tian et al.
International journal of molecular sciences, 20(5) (2019-03-13)
This study aimed to characterize the full-length cDNA of MARK4 in Sus scrofa, and evaluated its potential role in the regulation of lipid accumulation in pig placental trophoblasts and analyzed signaling pathways involved, thereby providing insights into mechanisms for placental
Yukimasa Takeda et al.
Scientific reports, 10(1), 3775-3775 (2020-03-01)
Brown adipocytes coordinate systemic energy metabolism associated with the pathogenesis of obesity and related metabolic diseases including type 2 diabetes. We have previously reported chemical compound-induced brown adipocytes (ciBAs) converted from human dermal fibroblasts without using transgenes. In this study
Qiang Gao et al.
Biochemical and biophysical research communications, 552, 84-90 (2021-03-21)
Angiotensin II (Ang II), an important component of the renin-angiotensin system (RAS), plays a critical role in the pathogenesis of cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as a promising platform for studying

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