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一般說明
Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
消光系数： E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
應用
Ficin from fig tree latex has been used:
- in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
- for the digestion of eye tissue sections prior to immunostaining and immunofluorescence
生化／生理作用
无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。
單位定義
在 pH 7.0 和 37°C 下，当在最终体积为 10mL（1cm 光程）的溶液中测定酪蛋白的 TCA 可溶性产物时，一单位每分钟产生的 ΔA280 为 1.0。
外觀
2.0M NaCl 和 0.03M 半胱氨酸中的悬浮液 (pH 5.0)
準備報告
2 次结晶
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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实验方案
This procedure may be used for all Ficin products.
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