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Merck

F4165

Sigma-Aldrich

无花果蛋白酶 来源于无花果树乳胶

powder, ≥0.1 unit/mg solid

别名:

Debricin, Ficain, higueroxyl delabarre

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

fig tree (latex)

形狀

powder

比活性

≥0.1 unit/mg solid

分子量

23.8 kDa

儲存溫度

−20°C

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相关类别

一般說明

消光系数: E1% = 21.0 (280 nm)
pI: 9.0

生化/生理作用

无花果蛋白酶(Ficin)被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道,显色底物pGlu-Phe-Leu-p-硝基苯胺的 Km 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP(二异丙基氟磷酸酯)、TLCK(Na-对甲苯磺酰基-赖氨酸氯甲基酮)和TPCK(N-甲苯磺酰基-L-苯丙氨酸氯甲基酮)抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′)2 片段。

單位定義

一个酶活性单位是指在测量酪蛋白的TCA可溶性产物时,在终体积为10ml(1cm光路)、37°C、pH 7.0条件下每分钟产生1.0的ΔA280 所需的酶量。
该酶溶于1 M磷酸钾缓冲液中,pH 7.0 (0.25 mg/ml),得到澄清溶液。

基底

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价格

抑制劑

产品编号
说明
价格

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Robertson D Davenport et al.
Transfusion, 44(6), 849-852 (2004-05-26)
Cefotetan can cause severe immune hemolytic anemia that may persist long after the drug is discontinued. To study the binding of cefotetan to RBCs, patients who received cefotetan were followed and tested for the presence of antibody to cefotetan. Patients
V B Reddy et al.
The British journal of dermatology, 163(3), 532-535 (2010-05-25)
Bromelain, ficin and papain are cysteine proteases from plants that produce itch upon injection into skin. Their mechanism of action has not been considered previously. To determine the mechanism by which these proteases function. The ability of these proteases to
A Saito et al.
Biochemistry and molecular biology international, 46(1), 27-34 (1998-10-24)
Amino acid sequencing of the ficin-derived C-terminal fragments of alpha-1-antiproteinase (also called alpha-1-antitrypsin or alpha-1-proteinase inhibitor) prepared from rabbit plasma revealed the presence of the E isoform, which had been confirmed in the cDNA library in addition to the F
Sadami Ohtsubo et al.
Journal of agricultural and food chemistry, 53(13), 5218-5224 (2005-06-23)
On the basis of cDNA sequences, we found that the calli of rice encodes an amino acid sequence that shares 56% and 89% identity, respectively, with oryzacystatin-I and oryzacystatin-II. This sequence differs from that of oryzacystatin-II in the N-terminal region
J D Reid et al.
The Biochemical journal, 357(Pt 2), 343-352 (2001-07-06)
The possibility of a slow post-acylation conformational change during catalysis by cysteine proteinases was investigated by using a new chromogenic substrate, N-acetyl-Phe-Gly methyl thionoester, four natural variants (papain, caricain, actinidin and ficin), and stopped-flow spectral analysis to monitor the pre-steady

实验方案

This procedure may be used for all Ficin products.

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