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Merck

D5546

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Dulbecco′s改良Eagle培养基(DMEM)是Eagle基础培养基(BME)的改良版,含有更高浓度的氨基酸和维生素及其他添加成分。据报道,原本的DMEM配方开发用于小鼠胚胎细胞培养,含有1,000 mg/L葡萄糖。改为4,500 mg/L葡萄糖后,适合特定类型的细胞生长。这种改良已被证明能够改善培养基的稳定性。 

應用

Dulbecco′s改良Eagle′s培养基(低糖)已用于培养:

  • 人骨肉瘤细胞和HeLa细胞
  • 原代人间充质干细胞(hBMSCs)
  • 人胚肾细胞系

重構

补充0.584 g/L谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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