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Merck

D5921

Sigma-Aldrich

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏改良Eagle培养基(DMEM)是对Eagle基础培养基 (BME)的改良,含有四倍浓度的氨基酸和维生素以及其他补充组分。据报告首个原始DME配方已用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。4,500 mg/L葡萄糖的修饰对培养某些类型的细胞效果最佳。已证明DME可提高培养基的稳定性。

應用

杜氏改良Eagle低葡萄糖培养基已用于培养:
  • 结肠癌SW620活细胞
  • 人乳腺癌细胞系MCF-7(13)、KPL和MDA-MB-231
  • 哺乳动物和犬源性脂肪源性间充质干细胞

重構

补充 0.584 g/L L -谷氨酰胺。

其他說明

使用干细胞或细胞克隆的研究者通常更喜欢不含酚红的培养基。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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