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Merck

D2429

Sigma-Aldrich

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

别名:

DME, DMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基-低葡萄糖, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

濃度

10 ×

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏改良Eagle培养基(DME)是对Eagle基础培养基(BME)的改良版,含有高四倍浓度的氨基酸和维生素及其他添加成分。据报道,原本的DME配方适合用于小鼠胚胎细胞培养,含有1,000 mg/L葡萄糖。

應用

杜氏改良Eagle培养基(低糖)已用于:
  • 在类器官包埋时,中和胶原蛋白I溶液
  • 黑色素瘤细胞系培养
  • 癌组织样本保存
  • C2C12细胞分化培养基

重構

补充 3.7 g/L 碳酸氢钠,0.584 g/L L -谷氨酰胺,0.004 g/L 叶酸,以 1×。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Maria Guix et al.
Scientific reports, 6, 21701-21701 (2016-02-26)
Novel approaches to develop naturally-induced drug delivery in tumor environments in a deterministic and controlled manner have become of growing interest in recent years. Different polymeric-based microstructures and other biocompatible substances have been studied taking advantage of lactic acidosis phenomena
Yi Li et al.
Physiological genomics, 50(3), 158-168 (2018-01-18)
Folic acid supplements taken during pregnancy can prevent neural tube defects and other developmental abnormalities. Here, we explored the effects of folate supplementation on gene expression and DNA methylation during C2C12 differentiation. Based on the folic acid concentration, this study
Tejas Shah et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 526-534 (2016-10-04)
Folic acid (FA) and iron are essential supplements during pregnancy. Similarly effects of vitamin B12 (B12) inadequacy and high folate and low B12 status, on pregnancy outcome are available. However there are no mandatory recommendations for B12. There are many
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Scientific reports, 7(1), 1705-1705 (2017-05-13)
Folate, an essential micronutrient, is a critical cofactor in one-carbon metabolism for many cellular pathways including DNA synthesis, metabolism and maintenance. Folate deficiency has been associated with an increased risk of neurological disease, cancer and cognitive dysfunction. Dihydrofolate reductase (DHFR)
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Balneotherapy and spa therapy have been used in the treatment of ailments since time immemorial. Moreover, there is evidence to suggest that the beneficial effects of thermal water continue for months following the completion of treatment. The mechanisms through which

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