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D4545

Sigma-Aldrich

Taq DNA聚合酶 来源于水生栖热菌

with 10× PCR reaction buffer without MgCl2

别名:

Taq polymerase, Taq polymerase enzyme

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About This Item

CAS号:
9012-90-2
EC 号:
2.7.7.7 (BRENDA, IUBMB)
MDL號碼:
MFCD00166026
分類程式碼代碼:
12352204
NACRES:
NA.55

生物源

enzyme from bacterial (Thermus Aquaticus)

品質等級

200

重組細胞

expressed in E. coli

形狀

liquid

用途

sufficient for 1500 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions
 sufficient for 5000 reactions

特點

dNTPs included: no
hotstart: no

濃度

5 units/μL

技術

PCR: suitable

顏色

colorless

輸入

purified DNA

適合性

suitable for PCR and automated sequencing reactions

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

Taq聚合酶是一种热稳定的DNA聚合酶，以嗜热细菌水生栖热菌命名。 一种稳定的脱氧核糖核酸（DNA）聚合酶（最适温度为80°C）已从极端嗜热菌水生栖热菌中纯化出来。 该酶没有磷酸单酯酶，磷酸二酯酶和单链核酸外切酶活性。 该酶的最大活性需要所有四个脱氧核糖核苷酸和活化的小牛胸腺DNA。

應用

来自水生栖热菌的Taq DNA聚合酶已用于：
  • DNA提取过程（基因扩增和测序过程）
  • 基因分型
  • 聚合酶链式反应(PCR)，研究上皮中性粒细胞活化肽78 (ENA-78)和白细胞介素-8 (IL-8)的组成性产生
  • 通过常规PCR扩增原代内皮细胞的RNA

生化／生理作用

Taq聚合酶可催化寡核苷酸引物驱动的、DNA模板依赖性地将dNTP掺入互补DNA链中。 它具有5′至3′聚合酶和核酸外切酶活性。

特點和優勢

  • 在单独的试管中提供MgCl 2，以优化MgCl2。
  • 可以耐受反复加热至95 °C而不出现明显活性损失。

包裝

Taq DNA聚合酶具有两种形式，含MgCl2的优化型10倍反应缓冲液(D1806)，或不含MgCl2的10倍反应缓冲液加独立管装MgCl2以用于滴定（D4545）。 第二种形式可能是确定最佳扩增条件所必需的。
不含MgCl2的Taq DNA聚合酶10倍反应缓冲液 包含单独一管的25  mM MgCl2

其他說明

Taq DNA聚合酶是一种来自于嗜热菌水生栖热菌（ Thermus aquaticus）的特殊热稳定酶。 该酶的重组形式可在大肠杆菌（ E. coli）中表达。 经过SDS-PAGE分析显示，这种分子量为94kDa的蛋白质不含可检测水平的污染性核酸内切酶或核酸外切酶。 它具有5′→3′聚合酶和核酸外切酶活性。

單位定義

一个酶活性单位是指在74℃下在30分钟内将10 nmol总dNTP掺入酸性可沉淀DNA中所需的酶量。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：US 8,404,464和US 7,972,828。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。

相關產品

产品编号
说明
价格

危險聲明

H412

防範說明

P273 - P501

危險分類

Aquatic Chronic 3

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

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A Chien et al.
Journal of bacteriology, 127(3), 1550-1557 (1976-09-01)
A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the
Nick A Bersinger et al.
Fertility and sterility, 89(5 Suppl), 1530-1536 (2007-09-01)
To analyze the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8) by epithelial cells and the response of these cells to cytokine stimulation. In vitro study using eutopic endometrial tissue. University hospital. Cycling women undergoing laparoscopy
Anna Bobrowska et al.
PloS one, 6(6), e20696-e20696 (2011-06-17)
Huntington's disease (HD) is a progressive neurodegenerative disorder for which there is no effective disease modifying treatment. Following-on from studies in HD animal models, histone deacetylase (HDAC) inhibition has emerged as an attractive therapeutic option. In parallel, several reports have
Ryan E Davey et al.
Stem cells (Dayton, Ohio), 24(11), 2538-2548 (2006-07-11)
Highly ordered aggregates of cells, or niches, regulate stem cell fate. Specific tissue location need not be an obligatory requirement for a stem cell niche, particularly during embryogenesis, where cells exist in a dynamic environment. We investigated autoregulatory fixed-location-independent processes
Rita Horvath et al.
Brain : a journal of neurology, 129(Pt 7), 1674-1684 (2006-04-20)
Mutations in the gene coding for the catalytic subunit of the mitochondrial DNA (mtDNA) polymerase gamma (POLG1) have recently been described in patients with diverse clinical presentations, revealing a complex relationship between genotype and phenotype in patients and their families.
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